ABSTRACT
Spinal cord injury is a significant cause of motor dysfunctions. There is no definite cure for it, and most of the therapeutic modalities are only symptomatic treatment. In this systematic review and meta-analysis, the effectiveness of stem cell therapy in the treatment of the spinal cord injuries in animal models was studied and evaluated. A systematic search through medical databases by using appropriate keywords was conducted. The relevant reports were reviewed in order to find out cases in which inclusion and exclusion criteria had been fulfilled. Finally, 89 articles have been considered, from which 28 had sufficient data for performing statistical analyses. The findings showed a significant improvement in motor functions after cell therapy. The outcome was strongly related to the number of transplanted cells, site of injury, chronicity of the injury, type of the damage, and the induction of immune-suppression. According to our data, improvements in functional recovery after stem cell therapy in the treatment of spinal cord injury in animal models was noticeable, but its outcome is strongly related to the site of injury, number of transplanted cells, and type of transplanted cells.
Subject(s)
Cell- and Tissue-Based Therapy , Contusions , Models, Animal , Spinal Cord Injuries , Spinal Cord , Stem Cell Transplantation , Stem CellsABSTRACT
Introduction: SOX9 is a transcriptional activator which is necessary for chondrogenesis. SOX6 are closely related to DNA-binding proteins that critically enhance its function. Therefore, to carry out the growth plate chondrocyte differentiation program, SOX9 and SOX6 collaborate genomewide. Chondrocyte differentiation is also known to be promoted by glucocorticoids through unknown molecular mechanisms
Methods: We investigated the effects of asynthetic glucocorticoid, dexamethasone [DEX], on SOX9 gene expression in chondrocytes
Results: SOX9 mRNA was expressed at high levels in these chondrocytes. Treatment with DEX resulted in enhancement of SOX9 mRNA expression. The DEX effect was dose dependent [0·5 nM and 1 nM]
Conclusion: RT-PCR analysis revealed that DEX also enhanced the levels of SOX9 expression. It was observed that DEX had enhancing effect only on SOX9 the expression level was low for SOX6. It can thus be concluded that chondrocyte differentiation can be promoted by DEX via SOX9 enhancement
ABSTRACT
Introduction: Multiple sclerosis [MS] is a disease of the immune system: it attacks the myelin around the axons and leaves them exposed. Destruction of myelin weakens the electrical conduction of ions and thus leads to a lack of communication in the nervous system
Methods: In the present study, we constructed recombinant plasmid and then transformed to E. coli cell. The colonies containing plasmid were selected by Colony PCR. Enzyme digestion and sequencing were utilized to approve the accuracy of the extracted plasmid of these clones. Recombinant plasmid transfect in to mesanchymal stem cells
Results: Plasmid was verified correctly. After transfection, the transcription of MOG gene and the expression of MOG protein were proved by RT-PCR, western blotting and Elisa
Conclusion: Plasmid was constructed correctly and mesenchyme stem cells were successfully transfected by transfection and protein can be expressed well, setting a proper foundation for the future studies on the transplantation of gene modified mesanchymal stem cells in order to promote Multiple sclerosis
ABSTRACT
Cloning and expression in the cells is one of the most important basic techniques to study a specific protein in molecular biology. Among gene transfer methods, non-viral methods are less expensive, easier and safer to be implemented. NT4 is an important gene with miscellaneous clinical applications. This gene is a neurotrophic that induce survival, development and function of neurons as well as inducing differentiation of progenitor cells to form neurons. The aim of this study was to construct and transfect NT4 gene, in order to use it in research and gene therapy. NT4 gene was subcloned in plasmid pcmvsport6, using PCR; then it was transformed in Escherichia coli bacteria strain DH5-alpha. The recombinant gene was extracted and restriction enzymes by NOTI, SAII. The fragment gene was legated into the PEGFP-N1 plasmid. Then by restriction enzymes HINDIII, the recombinant was detected through gel electrophosis. In order to ensure that recombinant gene is correct, it was sended for sequencing. Cell culture was prepared and the recombinant plasmid was transfected. SDS page and western blotting were used for detection of protein expression. Enzyme analysis showed that pcDNA had correct structure and sequencing, confirmed by 100% homology of the gene with reported alpha gene in Gene Bank. In the analysis of proteins isolated from transfected cells with SDS-PAGE, an approximately 45 kDa band was observed with monoclonal antibody which was confirmed by Western blot analysis. This plasmid is able to transform to Eukaryotic system and is suitable for evaluation of translation due to its proper structure